![Construction of a camelid nanobody phage display library and use of this library for screening of anti-SARS-CoV-2 nanobodies.](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-11/camelidnanobody.jpg?itok=pgcRoQ4O)
Construction of a camelid nanobody phage display library and use of this library for screening of anti-SARS-CoV-2 nanobodies.
A large-sized (diversity 7.5 x 1010) naive nanobody phage display library was constructed using B cells of over a dozen llamas and alpacas. Phages were screened for their high binding affinity for SARS-CoV-2 receptor-binding domain (RBD). Nanobodies expressed from the selected phages were further screened for their potency in neutralizing SARS-CoV-2 pseudovirus entry. The best performing nanobody was subjected to two rounds of affinity maturation.
Deputy Investigator
![Fang Li, Ph.D: University of Minnesota, coronavirus research center, spike protein, Midwest AViDD, University of minnesota, llama, lab leak, wuhan,](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/fang_li.jpg?itok=cJmiM6IZ)
Fang Li, Ph.D
![Midwest AViDD](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2023-01/umn_aviddwordmark.jpeg?itok=O2EK4gE9)
Deputy Investigator
![Michael Farzan, UF Scripps Biomedical Research, Lab Leak, Covid-19, Project 1: Small Molecule Entry Inhibitors of Pandemic Viruses](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/farzan.jpg?itok=GpO7mXkF)
Michael Farzan, Ph.D
![harvard](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2023-05/hms_logo_final_rgb_0.png?itok=_HPRzGgl)
![Choe Hyeryun, Project 1: Small Molecule Entry Inhibitors of Pandemic Viruses, UF Scripps Biomedical Research](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/choe.jpg?itok=l6m0OCro)
Hyeryun Choe, Ph.D: UF Scripps
![Stanley Perlman, M.D, Ph.D: University of Iowa, OSC, nanobody, nanosota-1, camelid, llama, sars, covid-19](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/stanlry_perlman_osc.png?itok=S_5EOA40)
Stanley Perlman, M.D, Ph.D: University of Iowa, OSC
![Matthew Aliota, Ph.D: University of Minnesota, OSC, aliota lab, animal testing, viruses, pandemic, virology, mutations](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/matthew_aliota_osc.png?itok=pYsMj9Z0)
Matthew Aliota, Ph.D: University of Minnesota, OSC
![Lijun Rong, Ph.D: University of Illinois Chicago, OSC, pandemic, virus, covid-19, antiviral, midwest avidd](/sites/midwestavidd.umn.edu/files/styles/folwell_full/public/2022-12/lijun_rong_osc.png?itok=DCtc7e_x)
Lijun Rong, Ph.D: University of Illinois Chicago, OSC